%0 Journal Article
%T Identification of an Antagonistic Bacterium Against Ralstonia solanacearun and Primary Study of Effect on Disease Resistance<br>一株番茄青枯菌拮抗菌的鉴定及抗病效果初探
%A YANG Song-Zhen
%A YAO Qing
%A SUN Xiao-Tang
%A ZHU Hong-Hui
%A <br>羊宋贞
%A 姚青
%A 孙晓棠
%A 朱红惠
%J 微生物学通报
%D 2007
%I 
%X On the basis of morphological, physiological and biochemical characters as well as sequence analysis of 16S rDNA, the antagonistic bacterium strain 3-1-16 was identified. Under optics microscope, the strain was bacilliform, G~ , stained evenly purple. Furthermore, many granulose structures were found in cell without parasporal crystal under EM observation. According to Biolog Bacteria Identified system, 3-1-16 strain has 98% similarity with Bacillus megaterium MO31. A 1443-bp 16S rRNA gene sequence was obtained from strain 3-1-16. The BLAST search of the GenBank database using this sequence showed its similarity to many species of Bacillus. It was 99.4% similar to the 16S rRNA gene sequence of Bacillus megaterium strain MO31 (GenBank accession No. AY553118.1) over 1437 bases. Phylogenetic tree of Bacillus 16S rRNA gene sequences showed that 3-1-16 strain was clusterred with three Bacillus megaterium. Based on those characteristics, strain 3-1-16 was identified as Bacillus megaterium GIMA 1.001. Glasshouse trials demonstrated that the control efficiency of 3-1-16 strain against tomato bacterial wilt disease was 81.3%
%K 16S rDNA<br>番茄青枯菌
%K 拮抗菌
%K 生理生化
%K 抗病效果
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4B288EE2828557DE&yid=A732AF04DDA03BB3&vid=339D79302DF62549&iid=94C357A881DFC066&sid=FF63C2887194B1FA&eid=AB152C2682BC8D3D&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=11