%0 Journal Article %T Isolation and Identification of Porcine Circovirus Type 2 Virus from a Domesticated Wild Boar
一株家养野猪源猪圆环病毒2型的分离与鉴定 %A WANG Hai-Yan %A GAO Song %A LIU Xiu-Fan %A
王海燕 %A 高 崧 %A 刘秀梵 %J 微生物学通报 %D 2008 %I %X The nucleic acid fragment of porcine circovirus type 2(PCV2)was amplified using PCR from the tissues of a domesticated wild boar with suspected postweaning multisystemic wasting syndrome (PMWS). The sample were then inoculated into the Dulac cells and passaged for 8 generation. The 4 nucleic acid fragments covered the complete genome for PCV2 were obtained by over-lapping PCR and sent to sequence. The sequence of genome was compared with other 9 strains originated from piglets in the same area. The result showed that these strains were in high homology. %K Domesticated wild boar %K Porcine circovirus type 2 %K Isolation %K Identification
家养野猪 %K 猪圆环病毒2型 %K 分离 %K 鉴定 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4E81A50CCA0D3415CA215CD2BFF61394&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=708DD6B15D2464E8&sid=2126E0EE2CFF9E30&eid=2614904FCB8D3A68&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=2&reference_num=14