%0 Journal Article
%T BOX-PCR profiling and biodiversity analysis of cultured bacteria in Huguangyan maar lake<br>湖光岩玛珥湖可培养浮游细菌的BOX-PCR图谱及生物多样性分析
%A JI Jian-D
%A WU Zao-He
%A ZENG Yong-Hui
%A DONG Ting-Ting
%A LU Yi-Shan
%A JIAN Ji-Chang
%A <br>纪建达
%A 吴灶和
%A 曾永辉
%A 董亭亭
%A 鲁义善
%A 简纪常
%J 微生物学通报
%D 2011
%I 
%X Maar Lake is a unique type of lake in geological structures, and little is known about microbial diversity in various Maar Lakes worldwide. In order to understand the community structures of culturable bacteria in Huguangyan Lake, a typical Maar lake in South China, BOX-PCR was introduced as a screening technique for bacterial isolates that were obtained from three water depths in winter and summer through the general R2A plates and in situ lake-water agar medium. The results indicated that the changes of bacterial abundance were consistent along water column and on the two types of medium: 5 m>1 m>13 m for summer and 1 m>5 m>13 m for winter, with Proteobacteria as the dominant bacteria followed by Actinobacteria, Firmicutes, and Bacteroides. BOX-PCR profiles for cultured bacteria on both mediums were similar, but their community structures were not identical. The bacterial diversity was higher in winter than that in summer, in consistent with the trends found in other similar primitive lakes. Our results show that using different media may have different limitations in cultivating microbes from natural environments. This study may contribute to the understanding of significant relationship between microbial diversity and environmental characteristics in unique Maar Lakes and to the isolation of novel functional bacteria.
%K Huguangyan
%K Maar lake
%K Bacterial diversity
%K Culture-dependent
%K BOX-PCR<br>湖光岩
%K 玛珥湖
%K 细菌多样性
%K 可培养性
%K BOX-PCR
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3455DCEB100283872BC15AE264D5925B&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=DF92D298D3FF1E6E&sid=BB44F42BE8AE7430&eid=E6D5A068841F33F3&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=32