%0 Journal Article
%T CLONING AND SEQUENCING ANALYSIS OF GINGIPAIN K OF PORPHYROMONAS GINGIVALIS<br>牙龈卟啉菌牙龈蛋白酶K的基因克隆和序列分析
%A ZHANG Feng-Qiu
%A YANG Lian-Jia
%A WU Zhi-Fen
%A YANG Ju-Cai
%A <br>张凤秋
%A 杨连甲
%A 吴织芬
%A 杨聚才
%J 微生物学通报
%D 2004
%I 
%X The desired DNA product of KGPcd and KGP-hag was obtained from the total DNA of Porphyromonas gingivalis by PCR with two pairs of gene specific primers. The segment of KGPcd and KGP-hag (about 1.5kb and 1.6kb) was inserted into pGEM-T easy Vector. The double-stranded DNA of the postitive clone was analyzed by restriction endonuclease mapping and DNA sequenceing. The sequences of KGPcd and KGP-hag were consistent with those of the references appeared. The proteins of KGPcd and KGP-hag will be obtained for further study.
%K Porphyromonas gingivalis
%K Gingipain K
%K Sequencing and Characterization<br>牙龈卟啉菌
%K 牙龈蛋白酶K
%K 序列测定和分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3DCC5925DE4ABDF9&yid=D0E58B75BFD8E51C&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=9C65ADEB5990B252&eid=35FC3610259C2B32&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=5