%0 Journal Article %T Numerical Taxonomy and 16S rDNA PCR-RFLP of Rhizobial Strains Isolated from Psoralea corylifolia etc
分离自补骨脂等宿主根瘤的24株菌的数值分类和16S rDNA PCR-RFLP 研究 %A HAO Yun-Jie %A WANG Meng-Zhao %A LIU Lei %A HAN Su-Zhen %A
郝云婕 %A 王孟兆 %A 刘 磊 %A 韩素贞 %J 微生物学通报 %D 2008 %I %X 24 strains obtained from root nodules of Psoralea corylifolia, Pueraria lobata, and Campylotropis macrocarpa of Yunnan province were studied with numerical taxonomy and 16S rDNA PCR-RFLP. Results of numerical taxonomy indicated that all strains included 10 reference strains were divided into 3 groups at 84% similarity. Group III is an unknown group with no reference strains. Group I is slow-growing kind, and group II fast and middle-slow-grower. The dendrogram derived from 16S rDNA PCR-RFLP showed that all strains divided into five phylogenetic branches at the similarity of 70%. They are branches I and V with no reference strains, Agrobacterium-Sinorhizobium-Rhizobium, Mesorhizobium and Bradyrhizobium. Not all results of numerical taxonomy are accord with 16S rDNA PCR-RFLP, and 2 strains at the same group with A. tumefaciens IAM13129T. %K Rhizobium %K Numerical taxonomy %K 16S rDNA PCR-RFLP
根瘤菌 %K 数值分类 %K 16S %K rDNA %K PCR-RFLP %K 分离 %K 补骨脂 %K 宿主 %K 根瘤菌 %K 数值分类 %K rDNA %K 研究 %K Isolated %K Strains %K Taxonomy %K 分类结果 %K 分支 %K 系统发育 %K 树状图 %K 分析 %K 菌群 %K 可分 %K 水平 %K 相似性 %K 菌株 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=229ABC1F52DBD12B256AA42B135D5C0D&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=94C357A881DFC066&sid=5BCC7395A64B6B90&eid=A2671A7646B3E25B&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15