%0 Journal Article
%T An Improved DAS Dot ELISA Method for Detection of Acidovorax avenae subsp.citrulli<br>改良DAS-Dot-ELISA检测西瓜细菌性果斑病菌
%A XIONG Liang-Bin
%A LIU Qing
%A WANG Tian-Chang
%A GAO Li-Ping
%A WANG Jing
%A LIU Shu-Tong
%A SONG Rui
%A SHI Ying-Bo
%A WANG Jun-Ping
%A WEN Zhao-Hui
%A <br>熊亮斌
%A 刘箐
%A 王天昌
%A 高丽萍
%A 王婧
%A 刘姝彤
%A 宋蕤
%A 施颖波
%A 王军平
%A 文朝慧
%J 微生物学通报
%D 2010
%I 
%X Based on nitrocellulose membrane, we optimized the blocking condition, the volume and concentration of coating antibody. Then, an improved Double Antibody Sandwich Dot Enzyme Linked Immunosorbent Assay (DAS-Dot-ELISA) for Acidovorax avenae subsp. citrulli, the casual agent of Bacterial fruit blotch of watermelon, was established. Use adding Disodium Ethylenediamine Tetraacetic Acid (EDTA) in nonfat dry milk treated with high temperature as blocking solution can effectively lower the background. Shaking gently can enhance hybridization and reduce the nonspecific binding. This improved DAS-Dot-ELISA assay detected Acidovorax avenae subsp. citrulli as low as 1.9 × 105 CFU/mL quickly and economically. On the detection of two batches seed sample, the germ-carrying rate with improved DAS-Dot-ELISA was 8.0%, 6.0% which was completely coincident with microwell plate ELISA. On the result of each seed, this improved method was coincided with microwell plate ELISA in 99%, showed a satisfactory prospect and provided a new rapid assay for the detection of Acidovorax avenae subsp. citrulli.
%K Acidovorax avenae subsp
%K citrulli
%K DAS-Dot-ELISA
%K Nitrocellulose membrane
%K Rapid detection<br>西瓜细菌性果斑病菌
%K 双抗体夹心Dot-ELISA
%K 硝酸纤维素膜
%K 快速检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=54BDC718C2FBF8AEFF44389089D1C7D6&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=F3090AE9B60B7ED1&sid=044F0D973C30117E&eid=A7E55B1B50263111&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=2&reference_num=0