%0 Journal Article
%T The Defective Potassium Uptake by TRK1 and TRK2 Mutant of Saccharomyces cerevisiae Using Homologous Recombination<br>酿酒酵母TRK1和TRK2钾吸收缺陷型菌株的构建
%A GUO Zhao-Kui
%A YANG Qian
%A YAN Pei-Qiang
%A WAN Xiu-Qing
%A YAO Quan-Hong
%A <br>郭兆奎
%A 杨 谦
%A 颜培强
%A 万秀清
%A 姚泉洪
%J 微生物学通报
%D 2008
%I 
%X In order to perform the regulation and functional study and the screening of potassium uptake genes, the high affinity K+ uptake genes TRK1 and TRK2 in Saccharomyces cerevisiae were replaced with URA3 and HIS3 alternately using the methods of homologous recombination. URA3 and HIS3 of S. Cerevisiae were used as marker gene, the defective K+ uptake TRK1 and TRK2 mutant of S. Cerevisiae were selected in the basic medium without Histidine and Uracil.
%K Saccharomyces cerevisiae
%K TRK1 mutant
%K TRK2 mutant
%K Homologous recombination<br>酵母
%K TRL1、TRK2吸钾基因缺陷型
%K 同源重组
%K 酿酒酵母
%K 钾吸收
%K 营养缺陷型
%K 菌株
%K Homologous
%K Recombination
%K Saccharomyces
%K cerevisiae
%K Mutant
%K Uptake
%K 生长
%K 恢复
%K 转运体基因
%K 拟南芥
%K 培养基筛选
%K 钾素营养
%K 基因缺失
%K 转运蛋白
%K 钾离子
%K 转化子
%K 标记基因
%K 组氨酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=1DE8BC2D2BAFC99D159C075B69EBB24F&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=0B39A22176CE99FB&sid=4AD6A901BD736716&eid=089D6A5945E87569&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=16