%0 Journal Article
%T DNA Extraction and Removing Humic Substance from Wetland Soil<br>湿地土壤微生物DNA提取及其脱腐技术
%A LI Jing-Yu
%A ZHAO Ji
%A BIAN Yu
%A WU Lin-Hui
%A YU Jing-Li
%A <br>李靖宇
%A 赵吉
%A 边玉
%A 武琳慧
%A 于景丽
%J 微生物学通报
%D 2010
%I 
%X DNA-based molecular biology techniques have widely been used as a powerful tool to understand the microbial community. In this paper, a new method to extract microbial genomic DNA from wetland soil was established, namely Calcium Chloride-SDS-Enzymatic. Calcium chloride rather than EDTA chelating agent was used to remove humic acids in the process of direct DNA extraction. The extracting time is less than 4 hours. In comparing with other two methods, this method is more efficient in removing humic acids from wetland soil, and the purity of extracted DNA is higher which can be applied to PCR amplification. It provides an efficient technology to extract and purify microbial genome DNA from soil for microbial ecological studies.
%K Wetland soil
%K DNA extraction
%K Humic acid
%K PCR amplification
%K Calcium chloride-SDS-enzymatic<br>湿地土壤
%K DNA提取
%K 腐殖酸
%K PCR扩增
%K 氯化钙-SDS-酶法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=CACDF4A35A711A6EE48A7F7AD2FFA077&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=5D311CA918CA9A03&sid=005F3549A8D9454D&eid=966760CE8B6E636C&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0