%0 Journal Article
%T CONSTRUCTION AND PRELIMINARY FUNCTION STUDY OFMURINE RECOMBINANT PHOSPHOLIPASE D2 ADENOVIRUS<br>鼠重组磷脂酶D2腺病毒的构建及功能的初步研究
%A HAN Li
%A WU Gui-Zhi
%A XU Zhen-Biao
%A XING Xu-Bing
%A JIA Ning WEI Hua
%A SUO Ji-Jiang
%A CHEN Shi-Ping
%A <br>韩黎
%A 吴桂芝
%A 徐振彪
%A 邢玉斌
%A 贾宁
%J 微生物学通报
%D 2004
%I 
%X To construct the murine recombinant phospholipase D2 adenovirus and study preliminarily its function in mammalian cells,Murine phosphatidylcholine-specific phospholipase D2 gene and its catalytically inactive mutant (K758R) was firstly sublconed from the eukaryotic expression vector pCGN into the shuttle vector pAdTrack-CMV encoding GFP (green fluorescent protein). The PmeI-linearized pAdTrack-mPLD2 was cotransformed with supercoiled adenoviral backbone pAdEasy-1 vector into BJ5183 E.coli for homologous recombination. Subsequently the recombinant mPLD2 adenoviral particles was successfully constructed. These particles infected human embryonic kidney 293 cells stably expressing M 3 muscarinic acetylcholine receptor (M 3 mAChR) with high efficiency detected by GFP. Meantime, significant PLD2 protein expression in 293 cells examined by immunoblotting has no effect on M3 mAChR-mediated PLD stimulation. However, infection by recombinant mPLD2 K758R adenoviruses inhibited the PLD stimulation induced by PMA, which was increased by its counterpart of wild-type mPLD2 adenoviruses. Our results demonstrated that homologous recombinant mPLD2 adenoviruses may be a powerful tool for studying further the important physiological role of PLD2 and protein kinase C might be involved specifically in PLD2 stimulation pathway.
%K Phospholipase D2
%K Adenovirus
%K Protein kinase C<br>磷脂酶D2
%K 腺病毒
%K 蛋白激酶C
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=F2E5D42E2D3EECC5&yid=D0E58B75BFD8E51C&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=7AA74D31F1FF2DCE&eid=C753EB8AC8F551B9&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=11