%0 Journal Article
%T Mutation and Selection of Transglutaminase Producing Strain by Atmospheric Pressure Glow Discharge Plasma<br>大气压辉光放电低温等离子体诱变选育谷氨酰胺转胺酶高产菌株
%A XIA Shu-Qin
%A LIU Long
%A ZHANG Dong-Xu
%A LI Jiang-Hu
%A DU Guo-Cheng
%A CHEN Jian
%A <br>夏书琴
%A 刘龙
%A 张东旭
%A 李江华
%A 堵国成
%A 陈坚
%J 微生物学通报
%D 2010
%I 
%X A novel mutation approach, namely, the atmospheric pressure glow discharge (APGD) plasma jet driven by a radio frequency (RF) power, was used to treat the spores of Streptoverticillium mobaraense 03-10 for the selection of transglutaminase (TGase) producer. The mutant with high TGase production was quickly screened according to the formation of color on the double-layered plate and the different appearances of colonies. The total mutation rate was over 42.8% and the positive mutant rate was 20.6%. The obtained mutant G2-1 has good genetic and morphological stability and TGase activity reached 2.73 U/mL, which was higher 82% than that of original strain.
%K Atmospheric pressure glow discharge plasma
%K Transglutaminase
%K Streptoverticillium mobaraense
%K Color-forming<br>大气压辉光放电低温等离子体
%K 谷氨酰胺转胺酶
%K 茂源链轮丝菌
%K 显色
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=DDBF228914BE485A3FDC19934369CAD0&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=708DD6B15D2464E8&sid=78FA856A8BE9CAB3&eid=4BFBB19F1A63A4F0&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0