%0 Journal Article
%T Key Factors of High-level Production of Polygalacturonate Lyase in Recombinant Pichia pastoris<br>碱性果胶酶在重组毕赤酵母中高效表达的关键因素研究
%A WANG Yun
%A HUA Zhao-Zhe
%A LIU Li-Ming
%A DU Guo-Cheng
%A CHEN Jian
%A <br>王 芸
%A 华兆哲
%A 刘立明
%A 堵国成
%A 陈 坚
%J 微生物学通报
%D 2008
%I 
%X The key factors on high-level polygalacturonate lyase (PGL) production in recombinant Pichia pastoris were investigated. In 250 mL shake flask, the optimal glycerol concentration, initial methanol concentration, methanol supplementation quantity, duration of induction, initial pH, medium volume were 40 g/L, 3.1 g Methanol/g DCW, 0.51 g Methanol/g DCW (in every 24 h), 72 h, pH6.0 and 30 mL, respectively. In 7 L fermentor, constant glycerol feeding, constant and DO-stat methanol feeding strategies were applied to enhance cell density and PGL production. Finally, the dry cell weight was 80 g/L, and the maximum yield of PGL was achieved to 217 U/mL, increased by 66.2% than that of flask level.
%K Polygalacturonate lyase
%K Recombinant Pichia pastoris
%K High-level expression
%K Methanol induction<br>碱性果胶酶
%K 重组毕赤酵母
%K 高效表达
%K 甲醇诱导
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=FFD095D937A48A2B213269BFBAED3984&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=38B194292C032A66&sid=F6A9FA47F5E81A49&eid=546F7227D3F3C7C1&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=13