%0 Journal Article
%T Screening and Identification of a New Elastase-producing Strain<br>新的产弹性蛋白酶菌株的筛选与鉴定
%A LIU Shuang-F
%A AN De-Rong
%A GOU Li-Xia
%A LI Na
%A <br>刘双发
%A 安德荣
%A 苟丽霞
%A 李 娜
%J 微生物学通报
%D 2009
%I 
%X The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw, and the selected strain was identified. The screening strategy included casein (skim milk) plate selecting and elastin (beef tendon) re-screening. And then, the morphological, physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally, the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree, the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus.
%K Bacillus
%K Elastase
%K Screening
%K Identification<br>芽孢杆菌
%K 蛋白酶
%K 筛选
%K 鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=FFCA3C62A21C99EADEE77458DE9A1518&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=9CF7A0430CBB2DFD&sid=5D2AEAEFE5867538&eid=9C959CAF55D6B1C2&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=13