%0 Journal Article
%T A New Method of Soil Bacterial DNA Extraction and T-RFLP Analysis for Diversity<br>土壤细菌DNA提取及多样性分析的T-RFLP方法
%A GE Yun-Ying
%A CHEN Song
%A HU Lan
%A TU Zheng
%A <br>葛芸英
%A 陈 松
%A 胡 兰
%A 凃 政
%J 微生物学通报
%D 2008
%I 
%X Obtaining soil bacterial DNA of good quality is a key step in soil bacterial ecology study. A quick, efficient, sensitive and stably method of DNA extraction from soil were established by combining strongpoints of two kits ( Soilmaster kit and DNA IQTM kit). In addition, the 16S rDNA gene and T-RFLP (Terminal restriction fragment length polymorphism) were used in the analysis of soil bacterial community diversity and the result show that T-RFLP is a powerful tool for bacterial community study.
%K Soil bacterial
%K Diversity analysis
%K DNA extraction
%K T-RFLP<br>土壤细菌
%K 多样性分析
%K DNA提取
%K T-RFLP分析
%K 土壤细菌
%K 提取
%K 多样性分析
%K 方法
%K Diversity
%K Analysis
%K DNA
%K Extraction
%K Bacterial
%K Soil
%K 研究
%K 群落多样性
%K Terminal
%K restriction
%K fragment
%K polymorphism
%K 序列
%K rDNA
%K 稳定
%K 快速
%K 结果
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=11DD3EA730CE4B879E211C94E504D090&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=6DE26652A1045643&eid=58F693790F887B3B&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=9