%0 Journal Article
%T Display of Superoxide Dismutase on the Surface of Lactococcus lactis by Use of the N-acetylglucosaminidase Anchor<br>利用N-乙酰葡糖胺糖苷酶在乳酸乳球菌表面展示超氧化物歧化酶
%A HUANG Xin-Feng
%A LI Xiao-Hua
%A LI Lin
%A <br>黄新凤
%A 李小华
%A 李林
%J 微生物学通报
%D 2010
%I 
%X In the present study, we utilized a previously characterized N-acetylglucosaminidase (AcmA) to develop a whole-cell catalyst of bacterial superoxide dismutase (SOD) in Lactococcus lactis. The truncated C-terminal domain (cA) and the full-length AcmA from L. lactis wild-type strain MB191, were used as the anchoring motifs to immobilize an Escherichia coli-derived SOD onto the surfaces of L. lactis ATCC11454 cells. The PCR-amplified cA fragment, the signal and the full-length sequences of acmA, were fused with sod to generate the recombinant ss-cA-sod and acmA-sod, respectively, followed by ligating into the expression vector pMG36K, yielding the recombinant strain MB193 (harboring the ss-cA-sod fusion gene) and MB194 (harboring acmA-sod), respectively. SDS-PAGE analysis showed that the substantial expression profile of the fusion enzymes cA-SOD and AcmA-SOD in the recombinant L. lactis MB193 and MB194, with the predicted Mr of 46 kD and 64 kD, respectively. The Mn-SOD activities of MB193 and MB194 cells were determined by using the standard xanthinoxidase assay procedure. It showed that MB193 and MB194 exhibited the higher whole-cell Mn-SOD activities by (2.63 ± 0.51) U/mL and (3.51 ± 0.64) U/mL, respectively, compared to the control strain MB192 by (1.53 ± 0.38) U/mL] that expressing the intracellular SOD. In addition, the recombinant MB194 cells exhibited the higher cell-surface display efficiency (by 56.4%) compared to MB193 cells (by 41.9%).
%K Cell surface display system
%K N-acetylglucosaminidase
%K Superoxide dismutase
%K Whole-cell catalyst<br>细胞表面展示系统
%K N-乙酰葡糖胺糖苷酶
%K 超氧化物歧化酶
%K 全细胞催化剂
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=AD103E91082F627C082490F96F5FEDB6&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=DF92D298D3FF1E6E&sid=D78CE6003C76BF9F&eid=443FBADBC753C4B7&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=0