%0 Journal Article
%T Screen of O2-tolerate Phenotype of Klebsiella oxytoca HP1 Mutants with High H2-evolving Activity by Selection with MNZ Combination O2<br>利用甲硝唑及外加氧方法筛选耐氧产氢Klebsiella oxytoca HP1突变菌株
%A WU Xiao-Bing
%A XU Hui-Juan
%A ZHI Xiao-Peng
%A XU Fang-Cheng
%A HU Zhong
%A LONG Min-Nan
%A <br>邬小兵
%A 徐惠娟
%A 支小鹏
%A 徐方成
%A 胡 忠
%A 龙敏南
%J 微生物学通报
%D 2008
%I 
%X Hydrogenases are key enzyme for bio-hydrogen production, most of them were rapidly inactivated by oxygen. It is important to bio-hydrogen production and hydrogen application that improve the O2-tolerance of hydrogenase. In this experiment, the hydrogen producing strain Klebsiella oxytoca HP1 was treated with 1% ethyl methanesulfonate(EMS) , the mutants with high O2-toleration ability were screened with 40mmol/L metronidazole (MNZ) and 21% oxygen. The H2-evolving activity of the first generation mutant HP1-A15 was increased 3.70 times than that of the wild-type (WT) under 15% oxygen. The H2-evolving activity of the second generation mutant HPA15-37 was enhanced 11 times than that of WT under the condition of 21% oxygen. The mutants HP1-A15 and HPA15-37 had steady heredity. These results suggest that MNZ and in addition oxygen is a good way to screen of O2-tolerate phenotype of facultativeanaerobe with high H2-evolving activity.
%K Hydrogenase
%K O2-tolerant
%K Metronidazole
%K Klebsiella oxytoca HP1
%K Mutagenesis<br>氢酶
%K 耐氧
%K 甲硝唑
%K 突变
%K Klebsiella
%K oxytoca
%K HP1
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=1DBD4936DE1C6A148BF9F1D69B5F550D&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=38B194292C032A66&sid=CEB25CE40F287A75&eid=27AE04498EC8FECF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=11