%0 Journal Article
%T Applications of BOX-PCR Technique in Diversity Study of Microorganisms<br>BOX-PCR技术在微生物多样性研究中的应用
%A YANG Feng-Huan
%A LI Zheng-Nan
%A JI Xi-Zhu
%A RAN Long-Xian
%A <br>杨凤环
%A 李正楠
%A 姬惜珠
%A 冉隆贤
%J 微生物学通报
%D 2008
%I 
%X In recent years, the molecular methods using short tandem repeats sequences in microbial genome to design primers and amplify the sequences have been continuously reported. The PCR based on the BOX elements is a simple, fast, highly reproducible method for comparing patterns of the amplified bands, and it was applied in studies on the classification of bacteria originally. Researchers found that it could be used as well for analysis of the genetic diversities of fungi and streptomyces using BOXA1R primer to amplify the BOX elements. This paper reviewed the characteristics and the general procedure of BOX-PCR fingerprinting. The system optimization of BOX-PCR fingerprinting for endophytic bacteria and BOX-PCR technology improvements are summarized. Its applications and prospect on diversity research of different microorganisms are discussed as well.
%K BOX-PCR<br>微生物
%K 多样性研究
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=7952F3BCF8135A35E43E7CB0E97972E7&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=5D311CA918CA9A03&sid=7CF64E95CEC38520&eid=F7C51083F4D893E5&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=30