%0 Journal Article
%T Isolation and Identification of a Glyphosate-resistant Yeasty Strain (ZM-1) and Its Optimal Growth and Degradation Traits<br>抗草甘膦酵母菌ZM-1的分离鉴定及其生长降解特性
%A TANG Ming-Qiang
%A YOU Min-Sheng
%A <br>汤鸣强
%A 尤民生
%J 微生物学通报
%D 2010
%I 
%X Using glyphosate as the sole selective pressure, a yeast strain ZM-1 was isolated by enrichment-domestication culture technique from cultivated soils in the suburb around Fuzhou, PR China. The strain was identified as Rhodotorula mucilaginosa based on its physiological, biochemical and 26S rDNA D1/D2 domain sequences analysis. Strain ZM-1 grew with glyphosate as sole carbon and nitrogen source. The maximum glyphosate tolerant concentration for ZM-1 was 50 g/L. Strain ZM-1 was cultivated for 7 d under the conditions that glyphosate concentration 1 g/L, temperature 30°C, and rotation speed 150 r/min with 85.38% glyphosate-degraded rate. With the basal salts medium, the optimal conditions for growth and glyphosate-degraded rate for the strain were as follow: initial glyphosate concentration 1 g/L, inoculating dose 4% (V/V), temperature 30°C, pH value 5.5-6.0 and medium contents 50 mL/250 mL, respectively. Strain ZM-1 could play an important role in bioremediation of glyphosate-contaminated environment, and may be a potential resource in the study of glyphosate-resistant GMO plant.
%K Glyphosate-degraded strain
%K 26S rDNA D1/D2
%K Phylogenetic analysis
%K Biodegradation traits<br>草甘膦降解菌
%K 26S
%K rDNA
%K D1/D2
%K 系统发育分析
%K 降解特性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=DF443033438F04B58A5CD4AC32B7555D&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=9CF7A0430CBB2DFD&sid=A41D5AE06B0DB66D&eid=113224FCB0E3054C&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0