%0 Journal Article
%T Studies on Establishing and Optimizing Conditions of Concentration Virus in Water Body
水体病毒浓缩方法的建立和优化研究
%A PAN Bao-Jin
%A LIU Jun-Yi
%A WEI Mei-Liang
%A
盘宝进
%A 刘军义
%A 韦梅良
%J 微生物学通报
%D 2005
%I
%X In this experiment six methods,calcium chloride(CaCl_(2)) precipitation,polyethylene glycol(PEG,pH7.0) precipitation,polyethylene glycol(PEG,pH11.5) precipitation,aluminum chloride(AlCl_(3)) precipitation,Amicon Utcra centrifugal filter devices and cellulose nitrate membrane were used to concentrate the vaccine poliovirus type 1(PV_(1)) added to water body;experimental conditions for concentration were selected and optimized.The results showed that two methods,CaCl_(2)and PEG(pH 7.0) precipitation were suitable for concentrating virus in large volumes of water while amicon utcra centrifugal filter devices for small ones.The virus recovery of the three methods reached a 100% rate.
%K Water body
%K Virus
%K Concentration methods
%K Optimizing experimental conditions
水体
%K 病毒
%K 浓缩方法
%K 条件优化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=30E1A2A66595AB68&yid=2DD7160C83D0ACED&vid=9971A5E270697F23&iid=B31275AF3241DB2D&sid=ECE8E54D6034F642&eid=D997634CFE9B6321&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=4&reference_num=8