%0 Journal Article
%T Genetic Diversity of Nematode Parasitic Bacteria Pasteuria spp.<br>线虫寄生菌巴斯德杆菌遗传多样性分析
%A LIAN Ling-Li
%A XIE Li-Yan
%A DUAN Yong-Ping
%A LIN Qi-Ying
%A WU Zu-Jian
%A <br>连玲丽
%A 谢荔岩
%A 段永平
%A 林奇英
%A 吴祖建
%J 微生物学通报
%D 2008
%I 
%X Pasteuria spp. was one of the highly potential biocontrol agents of plant-parasitic nematodes. Based on their 16S rDNA sequences, we developed simple PCR, PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) methods for rapid detecting the genetic diversities of Pasteuria spp. in root samples. Using simple PCR, we detected Pasteuria spp. from nine out of thirty nematode-infected samples collected from different crops in Fujian and Guangzhou of China. The genetic diversities of Pasteuria spp. in root samples were examined by PCR-RFLP and PCR-SSCP. Two of the five variant RFLP patterns were predominant among the clones when they were digested by restriction enzyme EcoHI. The results from PCR-SSCP analysis were consistent with those of PCR-RFLP and showed greater gene diversities. Twelve clones were chosen and sequenced. The results indicated the sequences of clones shared 97.8%~99.7% similar identity with those of previously reported P. penetrans, and the clones were further divided into one major clades and seven individual clades in the phylogenetic tree.
%K Pasteuria spp
%K 16S rDNA
%K PCR-RFLP
%K PCR-SSCP
%K Genetic diversity<br>巴斯德杆菌
%K 16SrDNA
%K PCR-RFLP
%K PCR-SSCP
%K 遗传多样性
%K 线虫
%K 寄生菌
%K 巴斯德
%K 杆菌
%K 遗传多样性分析
%K Bacteria
%K Nematode
%K Diversity
%K 遗传差异
%K 主要分支
%K 收录
%K GenBank
%K 进化关系
%K 系统
%K 同源性
%K 穿刺
%K 序列
%K rDNA
%K 显示
%K 测序分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=1A3AF5ED4C44F52D8C23FB488720BD6C&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=DF92D298D3FF1E6E&sid=C7A7E1E32985D389&eid=3F10405738B4D004&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=13