%0 Journal Article
%T Isolation and Characterization of a High Efficiency Aerobic Denitrifier<br>一株高效好氧反硝化菌的分离及特性
%A YANG Jun-Zhong
%A NI Yan
%A XU Shang-Ying
%A XU Ke-Han
%A LIU Yi
%A ZENG Li-Xia
%A LIU De-Li
%A <br>杨俊忠
%A 倪砚
%A 许尚营
%A 徐可瀚
%A 刘义
%A 曾丽霞
%A 刘德立
%J 微生物学通报
%D 2010
%I 
%X Twenty two bacterial strains with a capacity of denitrification were isolated from fish pond, of which eight strains have the high rate of denitrification while one strain named HS-N62 has the strongest effect of denitrification. The results showed that the denitrifying rate of strain HS-N62 reached 96% in 12 hours with initial nitrate nitrogen concentration of 140 mg/L and no nitrite nitrogen accumulation. The growth rate and characteristics of HS-N62 were further studied, and the results showed that the optimum growth temperature ranged from 30°C to 37°C, pH value from 6.0 to 8.0 and the optimal C/N ratio 10:1. Meanwhile strain HS-N62 could utilize a variety of carbon sources for growth. The most suitable denitrification condition for strain HS-N62 was confirmed by using a series of the orthogonal test. This denitrifying strain HS-N62 also has good ability of phosphorus removal, and the removal rate within 12 hours was up to 67.7% (at initial phosphate concentration 57 mg/L). Strain HS-N62 showed 99% 16S rRNA gene sequence similarity to many Pseudomonas strains. Based on the morphological characteristics, physiological and biochemical characteristics, the strain HS-N62 was identified as Pseudomonas sp.
%K Aerobic denitrification
%K Isolation and identification
%K Characteristic research<br>好氧反硝化
%K 分离鉴定
%K 特性研究
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=354AD2E7FFD4C71B7ECC3A36F55F37B2&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=708DD6B15D2464E8&sid=15863C3A31AE2538&eid=7C7B8F426F4B99BA&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0