%0 Journal Article
%T Isolation of Strains Producing Bio-demulsifiers and Comparison of Screening Methods<br>生物破乳剂产生菌的筛选及其方法研究
%A LIU Jia
%A HUANG Xiang-Feng
%A LU Li-Jun
%A WEN Yue
%A YANG Dian-Hai
%A ZHOU Qi
%A <br>刘佳
%A 黄翔峰
%A 陆丽君
%A 闻岳
%A 杨殿海
%A 周琪
%J 微生物学通报
%D 2008
%I 
%X Four screening methods, colorimetric assay, blood-plate hemolysis method, surface tension activity and oil spreading technique were introduced to isolate strains producing bio-demulsifiers from 6 different bacteria source samples. The results of various screening methods were evaluated in this paper. Seventeen demulsifying strains were obtained, which are qualified in demulsification test of kerosene model emulsions. Among them, 5 strains showed high demulsifying ability, achieving 70% plus demulsifying ratio within 24 hours. Petroleum-contaminated soil, excess sludge from biological process treating oilfield produced water and sludge from municipal wastewater treatment plant were the best among all tested sources. Due to the determination limit, the colorimetric assay and blood-plate hemolysis method are not competent to screen bio-demulsifiers strains. The measurement of surface tension and oil spreading method were easy but accurate methods to isolate bio-demulsifiers strains. Although demulsification test of model emulsion is an effective technique to target strains with the capability of breaking emulsions, it is sophisticated and time-con- suming. Thus it is recommended to use surface tension and oil spreading methods in pre-screening and validate the results in demulsification test with kerosene model emulsions.
%K Bio-demulsifiers
%K Surface tension
%K Oil spreading technique
%K Blood-plate hemolysis
%K Kerosene model emulsions<br>生物破乳剂
%K 表面张力
%K 排油圈法
%K 溶血圈
%K 煤油模型乳状液
%K 生物破乳剂
%K 菌的筛选
%K 方法
%K 研究
%K Methods
%K Screening
%K Comparison
%K Producing
%K Strains
%K 验证
%K 乳状液破乳
%K 初筛
%K 时间
%K 工作量
%K 模型
%K 生物表面活性剂
%K 检测范围
%K 存在
%K 溶血
%K 剩余污泥
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=BCEF8A790F743CEE7D4741F789D0E024&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=94C357A881DFC066&sid=480F17895A3DAA07&eid=B378CDC4AD4C09ED&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15