%0 Journal Article
%T Chitinase Gene Overexpression of Chaetomium cupreum in Pichia pastoris by Multi-site Mutations<br>角毛壳菌几丁质酶基因chi58多点突变及在毕赤酵母中的高效表达
%A WANG Yan-Jun
%A YANG Qian
%A <br>王艳君
%A 杨 谦
%J 微生物学通报
%D 2008
%I 
%X By using gene SOEing PCR, Arg (CGC) in the expression fragment of chitinase gene (chi58) from Chaetomium cupreum was mutated synonymously to Arg (AGA), which is a bias code of Pichia pastoris yeast. The expression plasmid pPIC9K-chi58A was constructed and transformed into GS115 strain through electroporation. The chitinase activity reached 101.71 U/mL±3.33 U/mL after induced with 120 h, which was the 3 fold of the original strain (31.83 U/mL±4.85 U/mL). Additionally, recombinant yeast showed good genetic stability after 10 cycle. SDS-PAGE analysis suggested that the weight of protein was 58 kD.
%K Chaetomium cupreum
%K Chitinase
%K Multi-site mutations
%K Pichia pastoris
%K Overexpression<br>角毛壳菌
%K 几丁质酶
%K 多点突变
%K 毕赤酵母
%K 高效表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=DE80A891F0A127241361B1B448DFD178&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=F3090AE9B60B7ED1&sid=E2EDBBF9B5CDD657&eid=B32C18974698DFB1&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=13