%0 Journal Article
%T Isolation, Identification and Degradation Characteristics of a DMP-degrading Strain<br>一株DMP降解菌的分离鉴定及其降解特性
%A JIN De-Cai
%A WU Xue-Ling
%A LIANG Ren-Xing
%A DAI Qin-Yun
%A WANG Yang-Yang
%A YANG Yu
%A <br>金德才
%A 吴学玲
%A 梁任星
%A 代沁芸
%A 王洋洋
%A 杨 宇
%J 微生物学通报
%D 2009
%I 
%X A bacterial strain which could grow well on the substrate of PAEs as the sole source of carbon and energy was isolated from contaminated sludge in the river of WeiFang in ShangDong province and it was designated as JDC-3. Based on the morphology, biophysical and biochemical properties as well as molecular characteristics, this isolate was preliminarily identified as Delftia sp.. A fragment of phthalate dioxygenase gene was successfully amplified from the genus of Delftia for the first time using a set of degenerate primers. Meanwhile, the degradation capability of JDC-3 was determined by HPLC using DMP as test substrate. The results showed that the optimal pH and temperature were at 7.0~8.0 and 30°C~35°C respectively. The degradation kinetics of JDC-3 was studied in different initial DMP concentration under optimal conditions. The results indicated that the degradation dynamic equation was ln C = - 0.06837 t + A when DMP concentration was lower than 300 mg/L, with half life of 12.48 h. The degradation rate decreased and half life of JDC-3 prolonged as the initial concentration kept on increasing.
%K DMP
%K 16S rDNA
%K HPLC
%K Degradation kinetics<br>邻苯二甲酸二甲酯
%K 16S
%K rDNA
%K 高效液相色谱
%K 降解动力学
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=36608CE5F6A5286BAB5CE0F66B9AF151&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=9CF7A0430CBB2DFD&sid=0E223208672FF713&eid=9F0564033BABD14F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=18