%0 Journal Article
%T A RT-HPLC Method for the Determination of Biogenic Amine Produced by Lactic Acid Bacteria<br>一种利用RT-HPLC分析乳酸菌产生物胺的方法
%A MENG Tian
%A TIAN Feng-Wei
%A CHEN Wei
%A ZHANG Hao
%A <br>孟甜
%A 田丰伟
%A 陈卫
%A 张灏
%J 微生物学通报
%D 2010
%I 
%X Biogenic amines are produced by some lactic acid bacteria of decarboxylase activities which are potential risk factors for lactic acid bacteria-fermented foods. In this paper, the biogenic amine-producing capacities of 61 lactic acid bacteria strains were analyzed on a decarboxylase medium. Then the methodology of RT-HPLC determination for biogenic amines in fermented broth and milk was studied. The biogenic amines in test samples were extracted by 10% trichloroacetic acid and then derived with benzoyl chloride. The HPLC analysis for the samples were conducted as the following condition: mobile phase was a gradient elution program of methanol and water, the flow rate was 0.8 mL/min. UV detector was used in the final de-termination on 254 nm. The results showed that biogenic amines were separated well on such condition. The method was in a linear way for the amines studied at given concentrations (R2 > 0.995). The average recov-ery ratios ranged from 97.92%?101.14% for all samples. The RSDs were less than 5%. It can be seen that the RT-HPLC method was rapid, stable, high sensitive. Combination of this HPLC method and microbial primary screening on a decarboxylase medium could evaluate the biogenic amine-producing capacity of lac-tic acid bacteria more accurately.
%K Lactic acid bacteria
%K Biogenic amine
%K Decarboxylase
%K RT-HPLC<br>乳酸菌
%K 生物胺
%K 脱羧酶
%K RT-HPLC
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=BBFCECC724912131381164CB2A880DE5&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=CA4FD0336C81A37A&sid=08A5DD34AD0F3625&eid=63F6C8363E1A77A6&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=2&reference_num=0