%0 Journal Article
%T Auto-inducing Expression Optimization of Mutant Human Glucagon-like Peptide-1 Fusion Protein in Escherichia coli BL21 (DE3)<br>人胰高血糖素样肽-1突变体融合蛋白在大肠杆菌中的自诱导表达优化
%A GU Juan
%A LAO Xun
%A JIN Ming-Fei
%A HUANG Jing
%A WU Zi-Rong
%A <br>顾娟
%A 劳勋
%A 金明飞
%A 黄静
%A 吴自荣
%J 微生物学通报
%D 2010
%I 
%X The auto-inducing expression of mutant human glucagon-like peptide-1 fusion protein in Escherichia coli BL21 (DE3) was studied. In order to increase the protein yield, we optimized both the auto-induction fermentation conditions and medium. The results showed that the optimal auto-induction medium is: tryptone 19.17 g/L, yeast extract 9.59 g/L, Na2HPO4 5.72 g/L, KH2PO4 5.48 g/L, (NH4)2SO4 2.66 g/L, NaCl 3.33 g/L, glycerol 2% (v/v), glucose 0.68 g/L, lactose 6.33 g/L, MgSO4 0.24 g/L. The genetically engineered strain was cultivated at 33°C, pH7, in a 100 mL flask containing 20 mL such medium, with the inoculum size of 1%, for 12 h. Then the yield of mutant human glucagon-like peptide-1 fusion protein reached 348.6 mg/L.
%K Mutant human glucagon-like peptide-1
%K Auto-induction
%K Optimization
%K Orthogonal design
%K Uniform design<br>人胰高血糖素样肽-1突变体
%K 自诱导
%K 优化
%K 正交设计
%K 均匀设计
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=93C7876EBDEFCA91EC4B3616C7DE722C&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=94C357A881DFC066&sid=C3E1E0E568E449E1&eid=FFFDFE15EEFD9410&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0