%0 Journal Article
%T Biotransformation of Neomycin by Mutational Strain of Streptomyces fradiae Mutagenized with Neutron Radiation<br>费氏链霉菌中子辐射诱变菌株对新霉素的生物转化
%A MA Lin
%A DA W
%A LIU Yun
%A SHI Guan-Ying
%A YANG Qian
%A YANG Cong-Jun
%A YANG Ling-Yun
%A ZHANG Xin-Gang
%A TAO Ke
%A HOU Tai-Ping
%A <br>马林
%A 达娃
%A 刘运
%A 史冠莹
%A 杨茜
%A 杨从军
%A 杨凌云
%A 张新刚
%A 陶科
%A 侯太平
%J 微生物学通报
%D 2010
%I 
%X A mutant of neomycin-producer Streptomyces fradiae was obtained from cultures mutagenized with neutron radiation. Utilizing the mutational strain which can not produce neomycin in normal culture, the biotransformation of neomycin was studied at different conversion conditions. The analysis results of fermentation broth by high performance liquid chromatography showed that the culture conditions such as the concentration of substrate, adding time of substrate, adding method of substrate, inoculation amount, the volume of medium, transformation time, carbon sources, nitrogen sources, pH of media and temperature had different influence on neomycin transformation. The transformation products of neomycin were isolated from a large scale fermentation broth by ion exchange chromatography and identified by thin layer chromatography. The antibacterial activity of four purified samples against Staphylococcus aureus, Erwinia carotovora and Pseudomonas solanacarum were tested by bioautographic analysis. All four samples showed obvious inhibitory activity against Staphylococcus aureus and Pseudomonas solanacarum, but only one sample had inhibitory effect against Erwinia carotovora.
%K Neomycin
%K Biotransformation
%K Irradiation mutagenesis
%K Streptomyces fradiae
%K Ion exchange chromatography
%K Antibacterial activity<br>新霉素
%K 生物转化
%K 辐射诱变
%K 费氏链霉菌
%K 离子交换层析
%K 抑菌活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=A3E116204F8C841948ED871F6A730265&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=9CF7A0430CBB2DFD&sid=AFB21040E5F48417&eid=B7AB8E33F0FC19ED&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0