%0 Journal Article
%T Cloning and Sequence Analysis of Mitogen-activated Protein Kinase Kinase Kinase Homolog from Volvariella volvacea (VV-MAPKKK)<br>草菇MAPKKK同源基因的克隆及序列分析
%A WANG Hong
%A BAO Da-Peng
%A CHEN Ming-Jie
%A CHEN Hui
%A FENG Ai-Ping
%A <br>汪虹
%A 鲍大鹏
%A 陈明杰
%A 陈辉
%A 冯爱萍
%J 微生物学通报
%D 2010
%I 
%X Two pairs of degenerate primers were designed according to the conserved amino acid sequences of several mitogen-activated protein kinase kinase kinases (MAPKKK) from basidiomycetes. Based on the conserved DNA sequence, which was obtained by nested degenerate PCR, a whole DNA sequence of MAPKKK of Volvariella volvacea (VV-MAPKKK) was obtained by blasting with Volvariella volvacea genome. VV-MAPKKK gene, containing 4 introns and codeing for a 1405 aa protein (VV-MAPKKK), is 4434 bp in length. The similarity of VV-MAPKKK compared with Hog-MAPKKK protein from Cryptococcus neoformans, Paracoccidioides brasiliensis and Cochliobolus heterostrophus is 58%, 57% and 56%, respectively. Phylogenetic analysis showed that VV-MAPKKK and Hog-MAPKKK protein homolog belong to the same clade. These data supported that VV-MAPKKK protein is a Hog-MAPKKK protein homolog from Volvariella volvacea.
%K Volvariella volvacea
%K Mitogen-activated protein kinase kinase kinase (MAPKKK)
%K Clone
%K Sequence analysis<br>草菇
%K 丝裂原活化蛋白质激酶激酶激酶(MAPKKK)
%K 克隆
%K 序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=2A468BEC647ECFF16B4B0E82906C9EB6&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=708DD6B15D2464E8&sid=C20D616EFC5754AE&eid=96BBFE99B0E6FBB3&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0