%0 Journal Article
%T ISOLATION OF ACIDOPHILIC AND ACIDODURIC STREPTOMYCETES USING DISPERSION AND DIFFERENTIAL CENTRIFUGATION APPROACH<br>应用分散和差速离心法分离嗜酸和耐酸链霉菌的试验及评价*
%A WANG Li-Ming HUANG Ying CUI Qing-Feng XIE Qiong ZHANG Ya-Mei LIU Zhi-Heng
%A <br>王黎明
%A 黄英
%A 崔庆锋
%A 谢琼
%A 张亚美
%A 刘志恒
%J 微生物学通报
%D 2003
%I 
%X Technological improvement for microorgnism isolation is important since isolation provides substantial materials for the exploitation of new microbial resources. In this study, a new approach, dispersion and differential cetrifugation (DDC), was applied in the isolation of acidophilic and acidoduric streptomycetes from 12 acid soil samples. Contrast with traditional method, the new approach yielded satisfying results with 2 - 20 times isolation efficiency and good selectivity. 45 representatives out of 249 streptomycetes isolates, which belonged to 12 color groups, showed morphology and cell wall type consistent with streptomycetes. The optimum pH range for their growth were between pH 4.5 - 5.5. It is proved that we succeeded in the rare-streptomycetes isolation using DDC approach.
%K Dispersion and differential centrifugation
%K Acidophilic streptomycetes
%K Acidoduric streptomycetes
%K Isolation<br>分散和差速离心
%K 嗜酸链霉菌
%K 耐酸链霉菌
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=998CAEE680F2F4F1&yid=D43C4A19B2EE3C0A&vid=340AC2BF8E7AB4FD&iid=B31275AF3241DB2D&sid=35FC3610259C2B32&eid=7AA74D31F1FF2DCE&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=10&reference_num=10