%0 Journal Article
%T The Apoptosis Induction of CT 26 Cells by Recombinant Clostridium difficile Toxin B<br>重组艰难梭菌毒素B对小鼠结肠癌CT26细胞的诱导凋亡作用
%A CHEN Min
%A CAO Yan-Fen
%A LI Shan
%A SUN Li-Hua
%A LIU Ting-Ting
%A WANG Ju-Fang
%A <br>陈敏
%A 曹延粉
%A 李杉
%A 孙丽华
%A 刘婷婷
%A 王菊芳
%J 微生物学通报
%D 2009
%I 
%X To investigate the induction of apoptosis of mouse colonic adenocarcinoma CT26 cells by recombinant Clostridium difficile toxin B (rTcdB), CT26 cells were exposed to different concentrations of rTcd B. Inhibition of cell proliferation was measured by MTT assay. The activation of Caspase 3 was measured by colorimetric method. Cell morphological analysis and flow cytometry were performed to confirm cell apoptosis. rTcd B inhibited the proliferation of CT26 cells in a time- and dose-dependent manner. Caspase 3 activity in CT26 cells was elevated remarkably after rTcd B exposure for 6 h, 12 h, 18 h or 24 h, as compared with the control group. Morphological changes were observed by fluorescence microscopy. The exposure of rTcd B to CT26 cells induced a time- and dose-dependent apoptotic cell death as determined by flow cytometry analysis. The results showed that recombinant Clostridium difficile toxin B induced apoptosis of CT26 cells.
%K Clostridium difficile
%K Recombinant toxin B
%K Apoptosis
%K CT26 cells<br>艰难梭菌
%K 重组毒素B
%K 细胞凋亡
%K CT26细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=E0A9A31FEAC93E8923C073E7BF88C80B&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=B31275AF3241DB2D&sid=6C679E11429D2211&eid=7FA386016DA95921&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=15