%0 Journal Article
%T Loop-mediated Isothermal Amplification Method for Detection of Nucleic Acids and Its Application in Food Safety Inspection<br>环介导等温扩增核酸技术及其在食品安全检测领域的应用
%A YANG Xiao-Juan
%A WU Qing-Ping
%A ZHANG Ju-Mei
%A ZHANG Shu-Hong
%A XU Xiao-Ke
%A <br>杨小鹃
%A 吴清平
%A 张菊梅
%A 张淑红
%A 徐晓可
%J 微生物学通报
%D 2010
%I 
%X Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method that amplified DNA with high specificity, efficiency and rapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity. The cycling reaction continues with accumulation of 109-1010 copies of target in less than an hour. The final products are stem-loop DNA with several inverted repeats of the target and cauliflower-like structures with multiple loops. A positive reaction would be shown as a ladder-like pattern in a gel electrophoresis analysis. Because of its simplicity, high sensitivity and specificity, the LAMP method has been widely applied to the field of food safety inspection.
%K Loop-mediated isothermal amplification
%K LAMP
%K Food safety
%K Inspection<br>环介导等温扩增
%K LAMP
%K 食品安全
%K 检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=1E09D768AEFAA88480106F359F1E87FE&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=5D311CA918CA9A03&sid=8EC0A96FD5EC3019&eid=603BC00D7DC5FEAC&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0