%0 Journal Article
%T Problems Analysis and Solution Strategies for Fluorescence Observation in VBNC Bacteria<br>VBNC细菌荧光观察技术问题分析及对策
%A LI Ying
%A DUAN Rui
%A QIAN Ai-Dong
%A <br>李影
%A 段锐
%A 钱爱东
%J 微生物学通报
%D 2010
%I 
%X A detection method of viable but non-culturable bacteria by fluorescence microscopy was established with SYTO9 and PI staining cell nucleus, ink instead of Iraq black, specimens fixed by hand. Problems on background fluorescence, cell aggregation, blur fluorescence image, low micropore film absorbability, unclear bacterial form, and fluorescence quenching were analyzed. Some solution strategies were suggested in order to offer help for researchers.
%K Viable but non-culturable in bacteria
%K Fluorescence microscopy
%K Problems analysis
%K Solution strategies<br>VBNC细菌
%K 荧光显微镜
%K 技术问题分析
%K 解决策略
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=FDFED04BBA7004234CE174BD662D5F6A&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=94C357A881DFC066&sid=B662A097B3823ADD&eid=FAC9F6C99E351D23&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0