%0 Journal Article
%T Methanogenic Diversity in an Anaerobic Digester with Chicken Feces<br>鸡粪沼气池产甲烷菌多样性
%A LANG Hui-Hu
%A YANG Hong-Jiang
%A ZHANG Yong-Gang
%A <br>郎会花
%A 杨洪江
%A 张永刚
%J 微生物学通报
%D 2010
%I 
%X Methanogenic diversity was characterized in an anaerobic digester with chicken feces by using a culture-independent approach. 50 clones of a 16S rDNA gene library were randomly selected and their sequences were phylogenetically analyzed. 46 of them (92%) was determined belonging to Methanogenium with similarities to Methanogenium marinum strain AK-1 between 99% to 100%; 3 clones (6%), KD525, KD526, and KD567, belonging to Methanoculleus with similarities 99% to Methanoculleus sp. dm2; one clone (2%), KD519, belonging to Methanocorpusculum with a similarity 99% to Methanocorpusculum bavaricum. Further more, the phylogenetic analyse of alpha subunit A of methyl-coenzyme M reductase (mcrA) clones were also performed. Gas chromatography assay showed that the content of formic acid was 28.85 g/L, 81.7% of the total volatile fatty acid of the fresh fermentation slurry; and the produced biogas from the digester was also determined, mainly composing of methane (55.5%), carbon dioxide (41.1%), and hydrogen (3.2%).
%K Anaerobic digester with chicken feces
%K Methanogen
%K Diversity<br>鸡粪沼气池
%K 产甲烷菌
%K 多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=7CFE7B1AAE4FFC7DBA3E7FC09C07649D&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=E158A972A605785F&sid=F27C5410DAC4BDE4&eid=1A3FE6F79953D0AF&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=3&reference_num=0