%0 Journal Article
%T Using Mini-Tn10 Transposon System to Research the Genes Involved in Biofilm Formation in Bacillus<br>利用mini-Tn10转座系统研究芽孢杆菌生物被膜形成相关基因
%A GAO Wei-Hu
%A HAO Jian-An
%A XIA Si-Yuan
%A XU Hai-Jin
%A BAI Yan-Ling
%A ZHANG Xiu-Ming
%A QIAO Ming-Qiang
%A <br>高卫华
%A 郝建安
%A 夏思源
%A 徐海津
%A 白艳玲
%A 张秀明
%A 乔明强
%J 微生物学通报
%D 2009
%I 
%X Biofilm has great influence for bacteria to resist the harm from outer environment. Its forming and development are controlled by many different genes. In this research, an insertional mutagenesis library of a wide type Bacillus amyloliquefaciens NK10.BAhjaWT was built via mini-Tn10 transposon system to find out genes involved in biofilm formation. Four such genes have been screened, in which, citB, citG, gpsA are all related to the energy metabolism. The function of another gene of yvfB is unknown. In a word, mini-Tn10 transposon system was proved to be efficient and stable in Bacillus.
%K Biofilm
%K Bacillus amyloliquefaciens
%K Mini-Tn10 transposon system<br>生物被膜
%K 解淀粉芽孢杆菌
%K mini-Tn10转座系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=0A3F84CFF2F61BE48C565FCF6E10EB09&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=38B194292C032A66&sid=546F7227D3F3C7C1&eid=56ADA1F0BCCD26B0&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=17