%0 Journal Article
%T Real-time Raman Spectrum of a Phenomenon in the Activation Process of Single Yeast Cell Cultured in Glucose Solution<br>一种酵母细胞生长现象的实时单细胞拉曼光谱观察
%A WANG Gui-Wen
%A YAO Hui-Lu
%A PENG Li-Xin
%A HE Bi-Juan
%A LI Yong-Qing
%A <br>王桂文
%A 姚辉璐
%A 彭立新
%A 何碧娟
%A 黎永青
%J 微生物学通报
%D 2007
%I 
%X Raman tweezers,a novel technique that combines Raman spectroscopy with optical tweezers,has the potential to become an effective tool for analysis single cell suspended in solution.To real-time investigate the biochemical proceedings in single cell,a Raman tweezers setup was used to trap a single instant active dry yeast cell and to acquire the real-time Raman spectrum of the trapping cell from dormancy to activation cultured in 2.0%(W/V)glucose.During the activating process,the intensity of bands derived from protein or lipids,such as 1000 cm-1,1445 cm-1,1655 cm-1,were not changed evidently.The intensity of the 1603 cm-1 band,which was considered as the signature of metabolic activity,was not changed evidently too.But the bands at 531cm-1,652cm-1,1053cm-1,1364 cm-1,which were assigned to trehalose,or glucose-base carbohydrates,were increased as the cell grew.This phenomenon,occurred in seven individual experiments of ten repeats using a batch of instant active yeast cells as tested cells,but did not occur when other batches of instant active yeast cells were tested.This peculiar process of adaptation was recorded by real-time Raman spectrum and the result indicates Raman tweezers is an effective tool for single-cell analysis.
%K Baker's yeast
%K Raman spectroscopy
%K Single-cell analysis<br>面包酵母
%K 拉曼光谱
%K 单细胞分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=DB72EEC1092C7343E387F483F54CEF3F&yid=A732AF04DDA03BB3&vid=339D79302DF62549&iid=B31275AF3241DB2D&sid=8DABBEB130EFF191&eid=0EFDF9DD0F63E842&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=16