%0 Journal Article
%T Construction of Two Standard Molecules for Detection of Tilletia indica Mitra and Tilletia walkeri Castl.<br>小麦印度腥黑穗病菌和黑麦草腥黑粉菌检测标准分子构建
%A LI Xiao-Jiao
%A WANG Zhong-Kang
%A ZHANG Gui-Ming
%A CHEN Zhi-Nan
%A YIN You-Ping
%A CHENG Ying-Hui
%A WANG Ying
%A XIANG Cai-Yu
%A MIU Jian-Kun
%A <br>李小焦
%A 王中康
%A 章桂明
%A 陈枝楠
%A 殷幼平
%A 程颖慧
%A 王颖
%A 向才玉
%A 缪建锟
%J 微生物学通报
%D 2010
%I 
%X Two standard molecules were constructed by molecular clone technology of Tilletia indica Mitra and Tilletia walkeri Castl.. The standard molecule for Tilletia indica Mitra contained two DNA fragments, one about 2297 bp DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. The standard molecule for Tilletia walkeri Castl. contained two DNA fragments, one about 2.3 kb DNA sequence from mitochondrial DNA, the other ITS sequence 710 bp. In this research, the two standard molecules were detected, the results show good specificity, homogeneity and stability which can satisfy the need of molecular detection of Tilletia indica Mitra and Tilletia walkeri Castl..
%K Clone
%K Molecular detection
%K Standard molecule<br>克隆
%K 分子检测
%K 标准分子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=5E3801028158EB2066FFD5D3A8BDAD41&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=F3090AE9B60B7ED1&sid=243AE565CB8D493C&eid=D9E4B4338A9AF29A&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0