%0 Journal Article
%T Cloning of VP1 and 3D Gene of Duck Hepatitis Virus 1 (DHV1) and Its Expression in Escherichia coli<br>I型鸭肝炎病毒VP1、3D基因克隆及其在大肠杆菌中的表达
%A KONG Liu-Wu
%A LUO Yu-Jun
%A ZHANG Gui-Hong
%A CHEN Jian-Hong
%A XU Xiao-Qin
%A LIAO Ming
%A KANG Yan-Mei
%A HE Yi-Min
%A <br>孔留五
%A 罗玉均
%A 张桂红
%A 陈建红
%A 徐小芹
%A 廖 明
%A 康艳梅
%A 何逸民
%J 微生物学通报
%D 2008
%I 
%X In this study, two special primer pair containing EcoR V and Xho I according to complete genome of duck hepatitis virus 1 (DHV1) were designed to amplify VP1 and 3D genes from cDNA of DHV1. The target genes VP1 and 3D were subcloned into PET32a vector digested by EcoR V and Xho I respectively. Then the recombinant plasmids were transfected into Escherichia coli BL21(DE3) for VP1and 3D expression. The bacteria containing PET32a-VP1 and PET32a-3D were collected and examined by SDS-PAGE and western-blotting. Result showed that the VP1 and 3Dprotein were expressed in E. coli and the amount of expression was higher. Molecular weight of the protein was 48 kD, 68 kD. The protein can be recognized by DHV1antibody. This study showed that the protein VP1 and 3D have antigenicity.
%K Duck hepatitis virus 1
%K VP1gene
%K 3Dgene
%K Cloning and expression<br>I型鸭肝炎病毒
%K VP1基因
%K 3D基因
%K 克隆表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=3D886F8C1D088EC7DF86C61E2B36354D&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=DF92D298D3FF1E6E&sid=305A58D956DD6BAF&eid=5DD55A2029F498FE&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0