%0 Journal Article
%T THE TECHNOLOGICAL CONDITIONS OF PRODUCING 2-KETO-LGULONIC ACID FROM D-GLUCOSE BY DIRECT FERMENTATION<br>D-葡萄糖串联发酵生产2-酮基-L-古龙酸的工艺条件
%A Zhang Gang Lan Xiande Fan xiaobin Han Zhihua
%A <br>张刚
%A 兰先德
%A 范小兵
%A 韩志华
%A 尹光琳
%A 马志方
%A 董文玲
%J 微生物学通报
%D 1992
%I 
%X 2-Keto-L-gulonic acid (2-KLG), the precurcor of L-ascorbic acid synthesis, was prepared directly from D-glucose by tandem fermentation. In the first step fermentation Erwinia sp. SCB 247 translated D-glucose to 2,5-diketo-D-gluconate (2,5-DKG), which accumenlated 180 mg 2,5-DKG per ml in the broth. In the second step fermentation Corynebacterium sp. SCB 3058 reduced 2,5-DKG to 2-KLG, which accumulated 35 mg 2-KLG per ml in the broth. This reductive fermentation was obtained under aerobic conditions by adding a hydrogen donor such as glucose.The average yield of five batches fermentation was 56.3 mol%, from D-glucose to 2-KLG in 10 L fermentor.
%K 2-Keto-L-gulonic acid
%K Fermentation
%K Vitamin C<br>2-酮基-L-古-酸
%K 维生素C
%K 发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=C4D6F7B353912D986EFCEE4F48784B3D&yid=F53A2717BDB04D52&vid=2A8D03AD8076A2E3&iid=0B39A22176CE99FB&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0