%0 Journal Article
%T Surface Display of Rhizomucor Miehei Lipase in Pichia pastoris Using Sed1p as an Anchor Protein and Its Application<br>利用Sed1p锚定蛋白在毕赤酵母表面展示米黑根毛霉脂肪酶及其应用
%A JIANG Feng-Chun
%A LIN Ying
%A YE Yan-Rui
%A <br>江逢春
%A 林影
%A 叶燕锐
%J 微生物学通报
%D 2010
%I 
%X We constructed a Rhizomucor Miehei Lipase(RML)-Displaying yeast whole-cell biocatalyst and applied it to methylesters synthesized from triglyceride and methanol. RML was fused with the Sed1p cloned from Saccharomyces cerevisiae to constructed plasmid pPIC9K-Flag-RML-Sed1. The plasmid was linearized and transformed into Pichia pastoris GS115 and Pichia pastoris recombinant strain GS115/pPIC9K-Flag-RML-Sed1 was obtained. Cell-surface display of the RML via Sed1p was confirmed by flow Fluorescence micrograph and flow cytometer. After incubated at 28°C for 48 h the hydrolytic activity of the GS115/pPIC9K-Flag-RML-Sed1 reached a plateau, 169.6 U/g (Dry cell weight). In nonaqeous media, the yield of 82.36% methylesters was obtained from triglyceride and methanol after 72 h using lyophilized RML displaying yeast whole cells.
%K Yeast cell-surface Display
%K Rhizomucor Miehei lipase
%K Whole-cell Biocatalyst<br>酵母表面展示
%K 米黑根毛霉脂肪酶
%K 全细胞催化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=18A47FD486C638FBE3211EBB4C2E1AEF&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=F3090AE9B60B7ED1&sid=33A7C6BD53CDAB47&eid=3DB657DD5E356007&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0