%0 Journal Article
%T Ability of Pig Lactobacilli Strains to Inhibit Salmonella typhimurium DT104 Adhesion and Invasion on Pig Intestinal Epithelial IPEC-J2 Cells<br>猪源乳酸杆菌对鼠伤寒沙门氏菌DT104在猪肠道上皮细胞IPEC-J2上粘附的影响
%A NI Xue-Qin
%A GONG Joshu
%A ZENG Dong
%A YU Hai
%A SI Weido
%A ZHOU Xiao-Qiu
%A <br>倪学勤
%A Joshua Gong
%A 曾 东
%A Hai Yu
%A Weido Si
%A 周小秋
%J 微生物学通报
%D 2008
%I 
%X The adhesion of 9 pig Lactobacillus strains on IPEC-J2 cell line and the effect of 5 Lactobacillus strains on the competitive, exclusive, displacement of adhesion and invasion of Salmonella typhimurium DT104 to IPEC-J2 cells were investigated. The 9 pig Lactobacillus strains exhibited dose- and strain-dependent adherence to intestinal epithelial cells by 0.1%~10%. The ability to inhibit the competitive adhesion of Salmonella typhimurium DT104 appeared to depend on the dose and Lactobacilli tested. Salmonella typhimurium adhesion was reduced above 80% by 109 CFU/mL K30, K67 and K16 strains. Adhesion percent of S. typhimurium DT104 was reduced 40%~70% by pre-treating the cells with 109 CFU/mL Lactobacillus prior to adhesion to IPEC-J2 monolayers, while invasion percent was reduced 23%~33% by 108 CFU/mL Lactobacillus. However, no inhibition of displacement was detected at low concentration of Lactobacillus, while 12%~84% displacement was detected with high concentration. Therefore, the observed inhibition of S. typhimurium adhesion and invasion to IPEC-J2 monolayers with Lactobacillus could be efficient for preventive or curative probiotic therapy in pigs with S. typhimurium disease.
%K Adhesion
%K Lactobacillus spp
%K Salmonella typhimurium
%K IPEC-J2 cells<br>粘附
%K 乳酸杆菌
%K 鼠伤寒沙门氏菌
%K IPEC-J2细胞
%K 猪源
%K 乳酸杆菌
%K 鼠伤寒沙门氏菌
%K 在猪
%K 肠道上皮细胞
%K 粘附率
%K 影响
%K Cells
%K Epithelial
%K Intestinal
%K Invasion
%K Adhesion
%K Salmonella
%K typhimurium
%K Strains
%K 猪沙门氏菌病
%K 有效防治
%K 筛选
%K 临床
%K 置换率
%K 高浓度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=9B7270CADAC404D2AA6BC129DE6291A6&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=DF92D298D3FF1E6E&sid=AF41770817DB8958&eid=E6E6318AC4BCBFDB&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=23