%0 Journal Article
%T Purification and Characterization of Laccase from Monodictys asperospera (Cooke & Massee) Ellis<br>丝孢菌Monodictys asperospera (Cooke & Massee) Ellis漆酶的分离纯化及其酶学性质
%A WANG Yi-Ning
%A ZHAO Guo-Zhu
%A ZHAO Yue-Ming
%A DI Xiao-Liang
%A XIE Xiang-Ming
%A <br>王祎宁
%A 赵国柱
%A 赵悦茗
%A 邸晓亮
%A 谢响明
%J 微生物学通报
%D 2009
%I 
%X A new wood-degrading fungus Monodictys asperospera (Cooke & Massee) Ellis with a high level of laccase production was chosen to study. This laccase was purified by ammonium sulfate precipitation, DEAE-cellulose and sephacryl S-300. Purification of about 8.1 fold was achieved with an overall yield of 5.7%. Its molecular weight was estimated to be about 77 kD. The optimum temperature and pH of the laccase activity were 55°C and 6.0, respectively. Kinetic studies of the laccase showed that the Km and the Vmax for using syringaldazine as substrate was 0.163 mmol/L and 0.194 mmol/(L·min), respectively. The carbohydrate content was 18.14%. In addition, it was found that laccase activity was significantly inhibited by Cu2+.
%K Hyphomycete
%K Laccase
%K Fermentation
%K Enzymatic characteristics<br>丝孢菌
%K 漆酶
%K 发酵
%K 酶学性质
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=58E60918533D7B7E774FD206497EEFE6&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=708DD6B15D2464E8&sid=7385F0F53E198CDC&eid=6FA25BADCDFACCA5&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0