%0 Journal Article
%T Selection of Yeast High in Lipids-yield by Fatty Acid Synthetase Inhibitor and Phosphoric Acid-vanillin Reaction<br>利用脂肪酸合成酶抑制剂和磷酸香草醛反应筛选高产油脂酵母菌
%A LI Ren-Min
%A WANG Ju-Fang
%A MA Shuang
%A YAN Ya-Ping
%A LI Wen-Jian
%A <br>李仁民
%A 王菊芳
%A 马 爽
%A 严亚平
%A 李文建
%J 微生物学通报
%D 2008
%I 
%X Rhodotorula glutinis (Fresenius) Harrison was irradiated with 80 MeV/u carbon ions. primary selection of yeast high in lipids-yield was performed with culture medium supplemented cerulenin, an inhibitor of fatty acid synthetase (FAS). The quantity of the lipids of selected strains was analyzed by phosphoric acid-vanillin reaction and traditional methanol-chloroform extraction methods. The results showed that the growth of Rhodotorula glutinis (Fresenius) could be inhibited efficiently by cerulenin. The inhibition rate was up to 98% at cerulenin concentration of 8.96×10-6 mol/L, which was considered suitable for mutant selection. Through the phosphoric acid-vanillin reaction, it was found that the quantity of the lipids was a positive linear relation with the OD at 530 nm. The positive mutant rate of primary selection reached to 65%. With the advantage of rapid and easy to perform, it can be considered as a good method to select yeast high in lipid-yield. By these methods, 2 mutant strains with lipid yield improved 90% compared with control was selected out.
%K Microbial lipids
%K Fatty acid synthetase (FAS)
%K Cerulenin
%K Phosphoric acid-vanillin reaction<br>微生物油脂
%K 脂肪酸合成酶
%K Cerulenin
%K 磷酸香草醛反应
%K 利用
%K 脂肪酸合成酶抑制剂
%K 过磷酸
%K 香草醛
%K 反应法
%K 酵母筛选
%K 高产
%K 油脂含量
%K 酵母菌
%K Reaction
%K Phosphoric
%K Inhibitor
%K Fatty
%K Acid
%K High
%K Yeast
%K 快速
%K 方法
%K 正突变率
%K 线性正相关
%K 光吸收
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=BA050DFAC7DA0171F9E24F03F17DB848&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=E158A972A605785F&sid=89906B64FE741834&eid=C50DA610856963EE&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=14