%0 Journal Article
%T Concise Plasmids for Antibiotic Resistance Cassette Recovery and in Vitro Recombination<br>便于抗性基因分离和分子重组的系列质粒的构建
%A LIU Da-Wei
%A NIU Dan-Dan
%A ZHANG Liang
%A SHI Gui-Yang
%A WANG Zheng-Xiang
%A <br>刘大伟
%A 牛丹丹
%A 张梁
%A 石贵阳
%A 王正祥
%J 微生物学通报
%D 2007
%I 
%X In gene manipulation, different selectable markers with various linkers are necessary. In order to get selectable markers directly, we constructed from pBlueScript SK(-) a series of particular plasmids, pSKsymKm, pSKsymBle, pSKsymEry, pSKsymHyg and pSKsymGm, each contains Kanamycin, Bleomycin, Erythromycin, Hygromycin or Gentamycin resistance cassette. By restriction enzyme digestion and gel extraction, any of five antibiotic resistance genes with specific ends can be conveniently obtained.
%K Selectable marker
%K Antibiotic
%K Recovery<br>选择性标记
%K 抗性基因
%K 分离
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=30A39F0BB520EC0A&yid=A732AF04DDA03BB3&vid=339D79302DF62549&iid=94C357A881DFC066&sid=97D084650B444622&eid=9DC3B794038556CA&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=14