%0 Journal Article %T Isolation and Identification of a Cellulose Degrading Bacterium and Its Cellulase Characterization
一株高效纤维素降解菌株的分离鉴定及其酶学性质 %A QIAN Lin %A ZHENG Qiao-Li %A FU Jin %A WANG Hui-Ping %A ZHENG Chun-Li %A LIU Jian-She %A
钱林 %A 郑巧利 %A 付瑾 %A 王慧萍 %A 郑春丽 %A 柳建设 %J 微生物学通报 %D 2010 %I %X A cellulose degrading strain DSH was isolated from the sediment of Dianshan Lake, Huangpu River, Shanghai. Basing on morphology, biochemical and physiological characterization, DSH was characterized as Bacillus cereus. A phylogenetic tree was constructed with the published 16S rRNA sequences of relative bacteria species. In the phylogenetic tree, the DSH strain is in a new branch which has the closest relative to Bacillus cereus strain JBE0004 with 99.78% sequence similarity. The generation of cellulose was closely correlated to its growth rate. The highest cellulose activity of DSH strain was 4.58 U/mL when grown at 37oC, pH 7.0 for 66 h. %K Cellulose %K Degrading %K 16S rRNA %K Bacillus cereus
纤维素酶 %K 降解 %K 16S %K rRNA %K 蜡状芽孢杆菌 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=84739952497ACF952C51A7A9979A5A96&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=E158A972A605785F&sid=B31172E46FD3509B&eid=005BD02EC967C423&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=4&reference_num=0