%0 Journal Article
%T Ultraviolet Mutagenesis Combined Chemostat Enrichment Method to Screen High-temperature Resistent and High-yield Lactic Acid Bacteria<br>紫外线诱变和恒化器培养筛选耐高温的高产乳酸菌
%A LU Ming-Bo
%A ZENG Xiang
%A ZHANG Li
%A LU Zheng-Dong
%A YU Long-Jiang
%A <br>鲁明波
%A 曾翔
%A 张力
%A 卢正东
%A 余龙江
%J 微生物学通报
%D 2010
%I 
%X This paper discusses the use of ultraviolet mutagenesis combined chemostat enrichment method to screen high-temperature resistent and high-yield lactic acid bacteria. Firstly, treated a strain of Lactobacillus rhamnosus with ultraviolet rays as mutagen, and then, before plate screening, used the chemostat continuous culture method to enrich high-temperature resistent and high-yield strains at 55oC. At last we obtained nine high-temperature strains. When fermented at 55oC, the highest production of L-Lactic acid was 62.9 g/L in 48 hours, which was 18.1 g/L higher than the starting strain. It was found that this method was more efficient than direct plate screening method by a comparing experiment, greatly reducing the workload of re-screening.
%K Lactic acid bacteria
%K Ultraviolet mutagenesis
%K High-temperature resisted
%K Chemostat Enrichment<br>乳酸菌
%K 紫外线诱变
%K 耐高温
%K 恒化器富集
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=60D9AE7B011C0776B68ADD1762C6E799&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=E158A972A605785F&sid=8B9DE198F96F5184&eid=08A784C493B89E54&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0