%0 Journal Article
%T Optimization of Saccharomyces cerevisiae sp. Strain by1.1b Culture Conditions for Efficient Biosynthesis of D-(-)-mandelate Dehydrogenase<br>酿酒酵母by1.1b产D-(-)-扁桃酸脱氢酶的发酵条件优化
%A YAN Fen
%A WANG Qian
%A LIN Zi-Lin
%A GUO Yang-Hao
%A <br>严 芬
%A 王 茜
%A 林子琳
%A 郭养浩
%J 微生物学通报
%D 2008
%I 
%X The culture conditions of Saccharomyces cerevisiae sp.strain by 1.1 b were optimized for the production of D-(-)-mandelate dehydrogenase which is useful for the asymmetric bioreduction of benzoylformate to form D-(-)-mandelate.The optimum medium(per liter)consistes of 60 g peptone,30 g maltose, 0.5 g MgSO_4,0.01 g ZnSO_4,1.0 g KCl.After optimization of the culture medium,the enzyme production in shake flasks is enhanced from 2.56 to 20.21 U/L.The optimum fermentation conditions were determined as follows:me...
%K D-(-)-mandelate
%K D-(-)-mandelate dehydrogenase
%K Saccharomyces cerevisiae sp
%K Fermentation condition<br>D-(-)-扁桃酸
%K D-(-)-扁桃酸脱氢酶
%K 酿酒酵母
%K 产酶条件
%K 酿酒酵母
%K 扁桃酸脱氢酶
%K 发酵温度
%K 条件优化
%K Saccharomyces
%K cerevisiae
%K Optimization
%K Strain
%K Biosynthesis
%K Efficient
%K Culture
%K Conditions
%K 培养时间
%K 时间进程
%K 细胞生长
%K 考察
%K 接种量
%K 装液量
%K 最佳
%K 摇瓶培养
%K 产量提高
%K 麦芽糖
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=15011914B8174406B1AE440AEB7BB61C&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=CA4FD0336C81A37A&sid=318E4CC20AED4940&eid=9FFCC7AF50CAEBF7&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=10