%0 Journal Article
%T Fermentation Characteristics of the Fast Conversion of Glycerol to 1,3-propanediol by Klebsiella pneumoniae HR526<br>克雷伯氏肺炎杆菌HR526快速合成1,3-丙二醇发酵特性研究
%A CHEN Zhen
%A ZHENG Zong-Ming
%A SUN Yan
%A HONG An-An
%A PENG Feng
%A LIU Can-Ming
%A LIU De-Hua
%A <br>陈 珍
%A 郑宗明
%A 孙 燕
%A 洪安安
%A 彭 枫
%A 刘灿明
%A 刘德华
%J 微生物学通报
%D 2009
%I 
%X The glycerol fed-batch fermentations with a novel isolated 1,3-propanediol production strains, designed Klebsiella pneumoniae HR526 were performed in 5 L B. Braun fermenter. The result indicated that 91.47 g/L 1,3-propanediol was produced in 30 h. The extracellular metabolic flux was calculated, indicating PDO flux reached the maximum value in the mid logarithmic growth phase, whereas lactic acid flux reached the maximum value in the stable phase. This article also analyzed the key enzyme activity of PDO synthesis, the maximum PDOR activity was reached in the mid of the logarithmic growth phase, the ratio of the GDHt/PDO and GDHt/GDH is rather higher in the logarithmic growth phase than in the stable, leading to 3-HPA accumulation during the exponential growth phase. The production of PDO is closely related to cell growth.
%K 1
%K 3-Propanediol
%K Specific enzyme activity
%K Extracellular metabolic flux<br>1
%K 3-丙二醇
%K 比酶活
%K 代谢通量
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=4941D237AEA9368748BEEDE45208688D&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=B31275AF3241DB2D&sid=C043CEFC9EA50900&eid=3FA2964ABBA4DDF2&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=0