%0 Journal Article
%T Comparison of RT-qPCR approaches for quantification of cyanobacteria in Lake Taihu<br>应用RT-qPCR技术定量检测湖泊水体中蓝藻方法的比较
%A PENG Yu-Ke
%A YUE Dong-Mei
%A WU Jun
%A XIAO Lin
%A YANG Liu-Yan
%A <br>彭宇科
%A 岳冬梅
%A 武俊
%A 肖琳
%A 杨柳燕
%J 微生物学通报
%D 2011
%I 
%X In the study, four methods used for quantification of cyanobacteria and Microcystis by SYBR Green I real-time quantitative PCR (RT-qPCR) were established based on the target genes, including the 16S rRNA gene of cyanobacteria, the phycocyanin and the 16S rRNA gene of Microcystis. Then the cultured M. aeruginosa and the environmental samples of Lake Taihu were evaluated by the established methods. Comparison between the new approaches and microscope counting indicated that RT-qPCR analysis could meet the requirement of algae bloom quantification and it was convenient, rapid and specific.
%K Lake Taihu
%K Cyanobacteria
%K Microcystis
%K real-time quantitative PCR
%K SYBR Green I<br>太湖
%K 蓝藻
%K 微囊藻
%K 实时荧光定量PCR
%K SYBR
%K Green
%K I
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=F94EF154262A95FC4256BFBBEF4CB700&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=E158A972A605785F&sid=E0172F1A638CE984&eid=98494933359B55EC&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=19