%0 Journal Article
%T Advances of Metagenomics<br>宏基因组学研究进展
%A HUANG Xun-Liu
%A HUANG Shi-Jie
%A GUO Li-Qiong
%A LIN Jun-Fang
%A <br>黄循柳
%A 黄仕杰
%A 郭丽琼
%A 林俊芳
%J 微生物学通报
%D 2009
%I 
%X It has evolved as a main limiting factor for the exploitation and application of microbial resources that more than 99% of microorganisms in natural environments cannot be cultivated so far by current isolation and culture methods. Metagenomics is an advanced methodology by means of extracting all microbial genomic DNAs in certain environmental habitat, constructing and screening metagenomic libraries to seek novel functional genes and/or biologically active compounds. It can overcome the advantages of isolation and cultivation procedures in traditional microbial method, and thus greatly broaden the space of microbial resource utilization. So it has become one of the powerful research tools for microbiology, biotechnology, soil and environmental sciences, and a new field of genetic engineering. This paper mainly reviewed the metagenomic methodology including genomic DNA isolation, library construction and screening strategies. And this review also outlined the up-to-date applications of metagenomics in various fields.
%K Metagenomics
%K Uncultured microorganism
%K Library construction
%K Library screening<br>宏基因组
%K 未培养微生物
%K 文库构建
%K 文库筛选
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=7CB6199761F98C33E8D2A20DCCFCFBB3&yid=DE12191FBD62783C&vid=933658645952ED9F&iid=DF92D298D3FF1E6E&sid=10343777FBBF55D5&eid=63025EFC6CC49145&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=72