%0 Journal Article %T Screening and Molecular Identification of Yeast Strain for Asymmetric Reduction of Acetophenone
不对称还原苯乙酮的酵母菌株筛选与分子鉴定 %A ZHU Dong-Mei %A CHEN Dan %A ZHENG Li-Li %A CHEN Hu %A YANG Zhi-Rong %A SUN Qun %A
朱冬眉 %A 陈 丹 %A 郑黎鹂 %A 陈 华 %A 杨志荣 %A 孙 群 %J 微生物学通报 %D 2008 %I %X Yeast strain YS6-2 with high stereoselectivity was screened from 145 strains when the asymmetric reduction of acetophenone to (S)-1-phenethylalcohol was chosen as the model reaction. With the concentration of acetophenone at 70 mmol/L, YS6-2 yielded (S)-alcohol at 26.8% conversion and enantiomeric excess reached up to 98.8%. Based on the analysis of 18S rDNA and 26S rDNA D1/D2 domain sequence, along with its general morphology, physiological and biochemical characteristics, YS6-2 was identified as Rhodotorula mucilaginosa. %K 18S rDNA %K 26S rDNA D1/D2
不对称还原 %K 苯乙酮 %K 酵母菌 %K 系统发育 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=DFAFCD67821E843DA12992CC1F58FE25&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=59906B3B2830C2C5&sid=245C5ED8B3BAFA8A&eid=0F2A4352C5EAE78D&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=18