%0 Journal Article
%T Screening and Molecular Identification of Yeast Strain for Asymmetric Reduction of Acetophenone
不对称还原苯乙酮的酵母菌株筛选与分子鉴定
%A ZHU Dong-Mei
%A CHEN Dan
%A ZHENG Li-Li
%A CHEN Hu
%A YANG Zhi-Rong
%A SUN Qun
%A
朱冬眉
%A 陈 丹
%A 郑黎鹂
%A 陈 华
%A 杨志荣
%A 孙 群
%J 微生物学通报
%D 2008
%I
%X Yeast strain YS6-2 with high stereoselectivity was screened from 145 strains when the asymmetric reduction of acetophenone to (S)-1-phenethylalcohol was chosen as the model reaction. With the concentration of acetophenone at 70 mmol/L, YS6-2 yielded (S)-alcohol at 26.8% conversion and enantiomeric excess reached up to 98.8%. Based on the analysis of 18S rDNA and 26S rDNA D1/D2 domain sequence, along with its general morphology, physiological and biochemical characteristics, YS6-2 was identified as Rhodotorula mucilaginosa.
%K 18S rDNA
%K 26S rDNA D1/D2
不对称还原
%K 苯乙酮
%K 酵母菌
%K 系统发育
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=DFAFCD67821E843DA12992CC1F58FE25&yid=67289AFF6305E306&vid=6209D9E8050195F5&iid=59906B3B2830C2C5&sid=245C5ED8B3BAFA8A&eid=0F2A4352C5EAE78D&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=18