%0 Journal Article
%T Strategies for Optimization Expression of Heterologous Protein in Pichia pastoris<br>毕赤酵母优化表达外源蛋白策略
%A YU Zhan-Qiao
%A MA Qing-Shan
%A ZHAO Long-Mei
%A ZHANG Ri-Jun
%A <br>余占桥
%A 马青山
%A 赵龙妹
%A 张日俊
%J 微生物学通报
%D 2010
%I 
%X Pichia pastoris is easy to genetically manipulate and can be grown to high cell densities, at the same time, P. pastoris is a eukaryote, and thereby has the potential for producing soluble, correctly folded recombinant protein with high yield, so the P. pastoris expression system is an ideal choice for the production of various heterologous proteins. However, at present not all the heterologous proteins can be successfully and efficiently expressed in P. pastoris, as a result, different proteins present different yield levels, bioactivity and stability. Here strategies for reducing proteolytic degradation and improving production of the expressed heterologous proteins are briefly summarized in terms of genetically factors and cultivation level.
%K Pichia pastoris
%K Heterologous protein
%K Optimization expression<br>毕赤酵母
%K 外源蛋白
%K 优化表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=BC35BF3EF3FC78788198B142C6184241&yid=140ECF96957D60B2&vid=42425781F0B1C26E&iid=DF92D298D3FF1E6E&sid=2418F98BB0851817&eid=9E5DBB98644FDC52&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=1&reference_num=0